How To Build Your Own Bazooka.pdf
The following antibodies and dilutions were used: anti-neurotactin 1:10 (BP106), anti-DN-Cadherin 1:20 (DN-Ex#7) (both Developmental Studies Hybridoma Bank); anti-Bazooka rabbit 1:500 (a gift from Dr Wodarz); Alexa 546-conjugated anti-rat Ig 1:500 (A11081), Alexa 546-conjugated anti-mouse Ig 1:500 (A11030) (both Molecular Probes, Eugene, OR, USA); and Cy5-conjugated anti-rat Ig 1:100 (112-175-102), FITC-conjugated anti-mouse 1:200 (115-095-100), Cy3-conjugated anti-rabbit Ig 1:200 (711-165-152) and Cy5-conjugated anti-mouse Ig 1:100 (115-175-166) (all Jackson ImmunoResearch, West Grove, PA, USA). For antibody labeling, standard procedures were followed . In brief, brains were dissected in 1 phosphate-buffered saline (PBS) pH 7.4 and fixed in 4% formaldehyde 30 minutes at room temperature or overnight at 4C. Fixed brains were washed with 1 PBS at least 30 minutes, and either stored in methanol at -20C or further processed. After at least three washes of 15 minutes each in 1 PBS with Triton X-100 0.1 to 0.3% (PBT), brains were incubated in 10% normal goat serum (GS0500; Capralogics Inc., Hardwick, MA, USA) for at least 1 hour at room temperature, followed by primary antibody incubation overnight at 4C. Brains were then washed at least 30 minutes in 1 PBT three times, incubated again in 10% normal goat serum for at least 1 hour, and left in secondary antibody for 2 hours at room temperature or overnight at 4C. Finally, brains were washed at least four times for 15 minutes each in 1 PBT, and mounted on glass slides in mounting medium (Vectashield; H-1000; Vector Laboratories, Burlingame, CA, USA). Brains were viewed under a confocal microscope (20 or 40 objective; MRC 1024ES microscope with Radiance 2000 and Laser Sharp 2000, version 5.2 build 824 software; all Bio-Rad, Hercules, CA, USA). Confocal sections were taken at 2 μm intervals for all preparations. We used Imagej1.41d software (National Institutes for Health, Bethesda, MD, USA) for image analysis and for generation of merged confocal sections. In cases where confocal stacks contained overlapping SATs along the Z-axis, Imagej4.1 was used to mask non-target lineages before generating a merged stack.
How to Build your Own Bazooka.pdf
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BEAMSWORDS AND BAZOOKAS is a tabletop role-playing game about mech pilots at the brink of World War 3. This core rule book includes steps for making characters, rules for tactical combat, and guidance for running the game as the gamemaster. It also includes a 10-episode campaign and setting details that give you a place to start your adventure.
As you may have noticed, these days haze is all the craze in the IPA world. After many, many test batches we are proud to present our version of this juicy style - Fruit Bazooka NE IPA. Only mildly bitter, the hop charges in this beer recipe kit create a blast of in-your-face flavors ranging from ripe mango, orchard fruits, melon, lime and passion fruit to subtle pine and floral notes. Fruit Bazooka is perfectly hazy thanks to the use of flaked oats, wheat malt, massive dry hopping, and a relatively non-flocculent yeast strain. The name itself was suggested by a homebrewer who witnessed our first tasting notes. And as it implies, this brew is bursting with an intense payload of citrus and tropical fruitiness.
Our marinated grilled chicken breast meals offer you or your family a delicious alternative for a healthy lunch, dinner or party. Probably the healthiest meals on the planet! Order it whole or chopped, with or without our homemade side orders
Popular coaxial cables for building this antenna are RG-8, RG-8x, RG-58, or RG-213. The RG-8 coax has a larger diameter, making an antenna with a larger bandwidth. An antenna made of RG-8 will also be heavier, especially if you are building an 80m antenna. Because of its weight, the connection between the tip and the coax might be a weak point. A heavy antenna might also be more challenging to install.
Because the coax parts of the antenna are matching stubs, they should be cut at 1/4λ. The length of these stubs will depend on the velocity factor of the coax. You can use your favorite search engine to download the datasheet of the coax you are using. The datasheet should indicate the velocity factor. For an "unknown" coax, you can use a modern Antenna Analyzer, a NanoVNA, or an Oscilloscope with a pulse generator to find the velocity factor.
Use the calculator above to calculate all the dimensions. Make sure you have the exact velocity factor for the type of coax you are using. Depending on how you want to mount your antenna, you might want to leave a certain length of wire at both ends. It will help you install an insulator. Having some margin to play with is also helpful to fine-tune the antenna to the exact frequency you want.
Remove 40mm (1.5inch) of the outer jacket at the center of the antenna. Cut the shielding in the center and separate it into two braids. One of the two braids will receive the center wire of your feed line, and the second braid the shield. Do not cut or damage the center dielectric of your coax.
At the desired length from the center of the antenna, remove the outer jacket and the shield, exposing the center dielectric of your coax. Leave a small quantity of braid exposed. It will be connected to the center conductor.
Carefully remove a small slice of the dielectric to expose the center conductor. Do not damage the center conductor. Damaging this conductor will structurally weaken your antenna. Solder the shield and the center conductor together. see the schematic below.
Seal all surfaces exposed to rain or moisture. Water getting inside the coax will ruin your antenna. You can use any product that blocks moisture from entering the coax. I use marine-grade heat-shrink tubing. These tubings contain glue that melts with heat. They will waterproof the connections, and they will also mechanically re-enforce that weak section of the antenna.
The antenna can cover the entire 6-meter band with a thin coaxial cable such as the RG-58. Building a Double Bazooka Antenna is as easy as making a dipole. The most challenging part of building this is removing the dielectric without damaging the center conductor. 041b061a72